DNA sensing by electrocatalysis with hemoglobin.

Electrocatalysis in DNA Sensors.

Electrocatalysis is often thought of solely in the inorganic realm, most often applied to energy conversion in fuel cells. However, the ever-growing field of bioelectrocatalysis has made great strides in advancing technology for both biofuel cells as well as biological detection platforms. Within the context of bioelectrocatalytic detection systems, DNA-based platforms are especially prevalent....

An Amperometric Nitrite Biosensor Based on the Bioelectrocatalysis of Hemoglobin Incorporated in Sol-gel Film

A highly sensitive, fast and stable biosensor for determination of nitrite was developed using hemoglobin immobilized on a poly (N-isopropylacyamide-co-3methacryloxypropyltrimethoxysilane) (PNM) modified glass carbon electrodes. The matrix provided a biocompatible microenvironment for retaining the native structure and activity of the entrapped hemoglobin. Nitrite could be reduced by the electr...

Electrochemical Single Base Mismatch Detection Based on Charge Transport Through DNA at DNA-Modified Electrode Surfaces Improved sensitivity and discrimination with electrocatalysis

Cobalt Phthalocyanine Modified Electrodes Utilised in Electroanalysis: Nano-Structured Modified Electrodes vs. Bulk Modified Screen-Printed Electrodes

Cobalt phthalocyanine (CoPC) compounds have been reported to provide electrocatalytic performances towards a substantial number of analytes. In these configurations, electrodes are typically constructed via drop casting the CoPC onto a supporting electrode substrate, while in other cases the CoPC complex is incorporated within the ink of a screen-printed sensor, providing a one-shot economical ...

Electrodeposited Graphene and Silver Nanoparticles Modified Electrode for Direct Electrochemistry and Electrocatalysis of Hemoglobin

By using a 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the working electrode, graphene (GR) nanosheets and silver nanoparticles (Ag NPs) were step by step electrodeposited on the surface of the CILE with potentiostatic method. The fabricated Ag/GR/CILE was used as a new platform for protein electrochemistry and hemoglobin (Hb) was immobilized on its surfa...